Manioc : Portail | resultats 1 - 20 pour la recherche: Aguilar, Laura

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

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Brisas de Cuba
Auteur(s) : Ponce de León, Néstor (1837-1899) Valdez Aguirre, Fernando De la Huerta, Santiago
Année de publication :

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Long-term decline of the Amazon carbon sink
Auteur(s) : Brienen, R J W Phillips, O L Feldpausch, T R Gloor, E Baker, T R Lloyd, J Lopez-Gonzalez, G Monteagudo-Mendoza, A
Auteurs secondaires : University of Leeds Coll Life & Environm Sci ; University of Exeter Sci Technol & Med, Dept Life Sci, ; Queen Mary University of London (QMUL) Sch Marine & Trop Biol ; James Cook University (JCU) Prolongac Bolognesi Mze ; Jardín Botánico de Missouri Sch Geog & Environm, Environm Change Inst ; University of Oxford [Oxford] University College of London [London] (UCL) Prolongac Bolognesi Mze ; Jardín Botánico de Missouri

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Mer des Antilles et golfe du Mexique. Première partie, comprenant les côtes des Guyanes hollandaise et anglaise, le Vénézuela, la Nouvelle-Grenade, l'Amérique centrale, le Mexique et les Etats-Unis
Auteur(s) : Sallot Des Noyers, Alexandre-Étienne-Denis (1819-1893)
Année de publication :

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Almanach américain, ou Etat physique, politique, ecclésiastique et militaire de l'Amérique : ouvrage qui comprend les forces, la population, les loix, le commerce et l'administration de chaque province de cette partie du monde ; le tableau de ceux qui y figurent par leurs charges et par leurs dignités ; celui de la marine des peuples européens qui y ont possessions, et le nom des officiers qui sont employés dans cette partie de l'administration publique
Auteur(s) : Poncelin de la Roche-Tilhac, Jean-Charles (1746-1828)
Année de publication :

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